Diagnostic Tests Based on Human Basophils: More Potentials and Perspectives Than Pitfalls. II. Technical Issues

■ Abstract Cellular basophil activation tests (BAT) such as histamine or sulfi doleukotriene-release tests for allergy diagnosis have been available for some time, but expression of basophil-activation markers such as CD63 and CD203c detected by flcytometry has attracted particular attention in recent years. Not only the potential but also the possible pitfalls of fl ow-cytometric BAT have been stressed recently. Some authors have suggested that the technical problems are still such that BAT should only be performed in specialist laboratories. In an earlier review based on our clinical experience obtained over several years, we showed that, even using different protocols, reproducible and meaningful clinical results can be obtained. In this paper, we review the current knowledge in relation to several technical issues and show that fl ow-cytometric BAT already represents a major advance in the fi eld of in vitro allergy diagnosis. We conclude that there are no serious technical justifi cations for depriving allergic patients of clinically indicated BAT tests, which can be performed reliably by any laboratory with the appropriate experience in allergy diagnosis and fl ow cytometry.