Growth kinetics of the algae Scenedesmus sp., and Chlorella sp. and its nanoparticle synthesis, collected from southern coasts of Chennai, India

The microalgal samples were collected from rock ponds in kovalam and isolated using plating techniques and identified in Krishnamurthy Institute of Algology, Chennai, Tamil Nadu, India. Algal cells were grown in Bolds Basal Medium (BBM) and cultured for 14 days in 16:8 h in 24°C, after 14 days algal cells were pelleted by centrifugation. The growth Kinetics of the algae Scenedesmus sp., and Chlorella sp. were studied and the generation time was calculated. The bioreduction of Ag+ ions in solution was monitored using UV-visible spectroscopy from zero reading and double distilled water was used as blank. The samples were withdrawn at various time intervals and the absorbance was measured. The silver nanoparticles were synthesized using extracts of algal samples was confirmed by color changes and was characterized by UV-visible spectrophotometer; the UV-visible spectra showed a broad peak located at 450nm for silver nanoparticles. The nanoparticles were also characterized by Scanning Electron Microscopy (SEM), X- Ray Diffraction (XRD) and Fourier Transmission Infra-Red Spectroscopy (FTIR) analysis. Key words: Scenedesmus sp., Chlorella sp., Silver Nanoparticles, Growth Kinetics