Characterization of a novel p63/p73 interacting protein.

6 LIST OF PAPERS NOT DIRECTLY RELEVANT TO THE THESIS 7 INTRODUCTION 8 P53 FAMILY PROTEINS: CRUCIAL PLAYER IN CANCER AND DEVELOPMENT 8 p53 8 p63 11 p73 13 RELEVANCE OF PROTEIN-PROTEIN INTERACTIONS IN THE REGULATION AND FUNCTION OF P53-RELATED PROTEINS. 15 An in vitro approach identifies novel evolutionarily conserved interactors of the p53 family of proteins 17 C16ORF35 19 AIM OF THE STUDY 24 MATERIALS AND METHODS 25 RESULTS 29 C16ORF35 IS A NUCLEO-CYTOPLASMIC PROTEIN 29 ANALYSIS OF NUCLEAR C16ORF35. 30 Nuclear c16orf35 localizes on matrix attachment regions 30 c16orf35 binds p63 and p73 but not p53. 32 c16orf35 binds GATA-1 and repress its transcriptional activity 35 c16orf35 binds members of transcriptional repressor complexes 37 ANALYSIS OF CYTOPLASMIC C16ORF35. 39 Cytoplasmic c16orf35 localizes to stress granules and processing bodies ..... 40 Overexpression of c16orf35 induces Stress Granules formation 44 Overexpression of c16orf35 affects tumor cells growth 46