Retrospective analysis of relationship between progression and regression of dysplasia and existence of HPV DNA by in situ hybridization

: We investigated the relationship between the progression and regression of cervical dysplasia and the existence of HPV DNA. The materials we analyzed were 80 biopsied specimens obtained from 22 cases of dysplasia which were followed-up for a long time at our out-patient clinic, and 47 specimens of squamous cell carcinoma. Detection of HPV DNA was done by in situ hybridization with biotinylated HPV DNA probes types 6/11, 16/18 and 31/33/35. The analysis of several specimens obtained at long term follow-up showed that the dysplasia-progression group had a higher incidence and more frequent detection of HPV DNA than the regression group. In the progression group, HPV 16/18 were detected more frequently than HPV 31/33/35 at the dysplasia lesion, but HPV 6/11 was detected in neither the dysplasia nor the squamous cell carcinoma. Fourteen of the 47 cases of squamous cell carcinoma (29.8%) were positive for HPV DNA, and 13 of them (92.9%) were detected in the lesions of dysplasia adjacent to carcinoma in situ or invasive cancer. Comparison of the detection rate for HPV DNA was done in the 3 dysplastic areas, i.e. the dysplastic areas in the dysplasia progression group (A), dysplasia regression group (B) and in the area adjacent to squamous cell carcinoma (C). The detection rates for HPV DNA were 43.5% in A, 27.7% in C and 15.0% in B. From these results we drew the following conclusions: 1) The continuous existence of HPV DNA, especially type 16/18 in the dysplasia lesions, may progress from lesions to carcinoma in situ or invasive cancer.(ABSTRACT TRUNCATED AT 250 WORDS)