Separation and extraction method for cytomembrane microvesicles (MVs) and exosomes (EXs)

The invention relates to a separation and extraction method for cytomembrane microvesicles (MVs) and exosomes (EXs). The method is characterized by comprising the following steps that a, 3-10 ml of a peripheral blood sample is drawn and placed in an anticoagulation tube to be stored, the sample is diluted with phosphate buffer normal saline (PBS) by 2-4 times, and then centrifugation is carried out to obtain supernate plasma; b, the supernate plasma is subjected to centrifugal separation again, and sediment is MVs; c, ultra-centrifugation is carried out on the supernate plasma in the step b, and sediment is EXs. According to the method, a previous method adopting mono-specificity antibodies is broken through, and the specificity and sensibility of the extracted cell MVs and EXs are higher than those in the prior art. A specificity and sensibility direction is provided for a further study of the MVs and the EXs as biomarkers of diseases.