In-vivo imaging tracking system of mouse hepatitis coronavirus and application of tracing system

The invention discloses a LUC (Luciferase) tracing system of a mouse hepatitis coronavirus and application of the tracing system. The in-vivo imaging tracking system of mouse hepatitis coronavirus is used for recombining the mouse hepatitis coronavirus by replacing or inserting wild mouse hepatitis coronavirus genome RNA (Ribose Nucleic Acid); in the replacing process, any segment in a segment a in the wild mouse hepatitis coronavirus genome RNA is replaced with a segment b; in the inserting process, any locus in the segment a in the wild mouse hepatitis coronavirus genome RNA is inserted in the segment b; the segment a is RNA coded by a sequence l in a sequence table; and the segment b is RNA coded by a DNA (Deoxyribonucleic Acid) segment containing a caussia luciferase coding gene. According to the in-vivo imaging tracing system of the house hepatitis coronavirus and application of the tracing system disclosed by the invention, the MHV (Mouse Hepatitis Virus)-LUC and the wild virus can be used for research at the same time. Besides, the MHV-LUC can be used for greatly improving the virus monitoring flexibility due to an LUC signal amplifying effect, so that a new idea is provided for establishment of a low-dose virus subclinical infected animal model, and a new technical platform is provided for the application, research and the like of the coronavirus resistant drug screening.