黄连解毒汤对APP/PS1双转基因AD小鼠脑组织IL-6I、L-1β水平及SP、NFTs数量的干预作用

Objective To observe the influence of Huanglianjiedu decoction (HJD) on contents of interleukin-6 (IL-6) and interleukin-1β (IL-1β) and numbers of senile plaques (SP) and neurofibrillary tangles (NFTs) in brain of APP/PS1 double transgenic Alzheimer disease (AD) mice. To discuss the possible mechanism of AD treated by HJD and to compare the effect between HJD and hydrochloride acid donepezil. Methods After 7-month gavage treatment by high, middle and low doses of HJD or hydrochloride acid donepezil respectively, enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of inflammatory cytokines IL-6 and IL-1β in APP/PS1 double transgenic AD mice brain. Immunofluorescence staining was used to observe the numbers and morphology of SP and NFTs, and pathological changes of nerve cell nucleus in hippocampus area through fluorescence microscope. Results Compared with the control group, IL-6 levels and the percentage of denaturation of Neurons' nucleus (PNND) reduced in the hydrochloride acid donepezil group and the HJD groups (P＜0.01); the number of SP reduced in the hydrochloride acid donepezil group and the high, middle and low dose HJD group (P＜0.01, P ＜0.01, P＜0.01, P＜0.05); the formation of NFTs decreased in HJD groups and hydrochloric acid donepezil group (P＜0.01, P＜0.05). Compared with the hydrochloric acid donepezil group, IL-6 levels decreased in the high, middle and small dose HJD groups (P＜0.05, P＜0.01, P＜0.05); the number of SP reduced in the middle HJD dose group (P＜0.05); the formation of NFTs reduced in the middle dose group (P＜0.01); PNND reduced in the middle dose HJD group (P＜0.01). The number of SP was less in both the high and middle dosage HJD groups than in the small dose group (P＜0.01); the formation of NFTs was more in the high and small dose groups than in the middle dose group (P＜0.05, P＜0.01); the PNND increased in the high dose HJD group compared with the middle and small dosage HJD groups (P＜0.01, P＜0.05), and the PNND reduced in the middle dosage HJD group compared with the small dose group (P＜0.01). There were no significant differences in IL-6 levels among the HJD groups and IL-1β levels among all the groups (P＞0.05). Conclusions The neuronal cells could be protected by treating with HJD or hydrochloride acid donepezil. It seems that HJD was more effective than the hydrochloride acid donepezil in treating AD.