Valoración de la sobreexpresión proteica en pacientes con SAHS leve

espanolIntroduccion: El sindrome de apneas-hipopneas del sueno (SAHS) es una enfermedad frecuente y asociada a morbimor-talidad en estadios graves. Se postula que el SAHS puede tener una expresion proteica diferencial respecto a un grupo de pa-cientes sin SAHS. Se disena un trabajo para determinar si un estudio proteomico es capaz de identificar proteinas sobreex-presadas en enfermos con SAHS leve. Metodos: A los sujetos se les realizo una historia clinica, ex-ploracion fisica, bioquimica general, polisomnografia diag-nostica y estudio proteomico mediante iTRAQ (isobaric tags for relative and absolute quantification). Se incluyeron 11 pacientes con SAHS, indice de apneas-hipopneas (IAH) ≥ 5, cuyos re-sultados fueron comparados con un grupo sin SAHS (IAH 1.3) respecto al grupo control. Estas proteinas fueron la PRO0684 (gi|6855601), la immunoglobulina lambda de cade-na corta VLJ (gi|21669561) y el kininogeno HMW precursor (gi|68785). Las principales funciones y procesos biologicos en los que estan involucrados son la inflamacion, respuesta inmune y coagulacion. Conclusiones: Respecto a un grupo control, en los pacientes con SAHS se observan proteinas sobreexpresadas. Estas pro-teinas pueden ser de ayuda para el diagnostico o para aumen-tar el conocimiento en procesos fisiopatologicos subyacentes. No obstante, son datos preliminares, cuyo alcance debe esta-blecerse en estudios posteriores. EnglishIntroduction: The apnea-hypopnea sleep syndrome (AHSS) is a frequent illness, associated to morbidity and mortality in its most serious stages. It has been postulated that AHSS may have a di-fferential protein expression with regards to a group of patients without AHSS. A study has been designed to determine whether or not a proteomic study is able to identify the overly expressed proteins in patients with minor AHSS. Method: Full clinical background was noted for the subjects, as well as physical examination, general biochemical tests, diagnostic polysomnography and proteomic study using iTRAQ (isobaric tags for relative and absolute quantification). Included in the study were 11 patients with AHSS, an apnea-hypopnea index (AHI) ≥ 5, whose results were compared to a control group without AHSS (AHI 1.3) with regards to the control group. These proteins were PRO0684 (gi|6855601), short immunoglobulin lambda chain VLJ (gi|21669561) and the kininogen HMW precursor (gi|68785). The main functions and biological processes include inflammation, immune response and coagulation. Conclusions: With regards to the control group, patients with AHSS showed overly expressed proteins. These proteins may be helpful in the diagnosis or in increasing knowledge about un-derlying physical-pathological processes. Nevertheless, this data is preliminary, with scope of which must establish future studies.