An efficient temporary immersion system for micropropagation of hybrid hazelnut

An efficient protocol for the micropropagation of hybrid hazelnut (Corylus L.) was developed using a temporary immersion system (TIS) for the proliferation of shoots in a liquid medium. Growth and proliferation of shoots as well as the length and number of nodes in developing shoots were significantly improved by combining two forms of chelated iron: ethylenediamine-N,N′-di-(ortho-hydroxyphenyl) acetic acid (FeEDDHA), and FeSO4/EDTA (FeEDTA). The use of TIS with optimized concentrations of FeEDTA and FeEDDHA significantly increased the number of shoots, shoot height, leaf area, and chlorophyll content in all of the four cultivars tested, compared with a semi-solid medium. Proliferated shoots developed roots on a semi-solid medium containing 2.5 μmol/L indole-3-butyric acid and rooted plantlets acclimatized in greenhouse conditions with 80% survival.