Efeito da concentração inicial de frutose sobre a conservação a 5 °c do sêmen caprino
2012
Twenty-four goat semen samples were collected and divided into four aliquots, diluted with the citrate-egg yolk (CY), TRIS-egg yolk (TY) or industrialized coconut water with egg yolk (ICW-Y) extenders. The fourth aliquot was centrifuged to analyze fructose concentration and PLA2 activity on SP. The semen was stored at 5 oC and evaluated at times fresh, 2, 24 and 48 h, in each time was evaluated the vigor, sperm motility and total morphological alterations. The animals were divided into two groups: group I-fructose concentration >710 mg/dL and group II-fructose concentration <480 mg/dL. The ICW-Y extender presented inferior results. The effect of the group was observed only in the ICW-Y. CY and TY worked similarly in the two groups. No significant difference in the activity of PLA2 was found between the groups. However, ICW-Y should not be used for the storage of goat semen.
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