Effect of silencing Vav3 by siRNA on the invasion capability of Lewis lung cancer cells

OBJECTIVE: To observe the effect of small interference RNA(siRNA) mediated silencing of the vav3 on cell migration and invasion ability in Lewis lung cancer cells.METHODS: Chemically synthesized siRNA targeting Vav3(Vav3-163)was transfected into Lewis lung carcinoma cells.Western blotting were applied to detect the protein expression of Vav3.The migration and invasion of Lewis lung carcinoma cells in viro was measured by Transwell chamber assays.RESULTS: SiRNA of mouse Vav3-163 could inhibit the expression of Vav3.The protein level of Vav3 was decreased in Lewis lung cancer cells which transfected with Vav3-163(0.112±0.005) compared with the Lewis lung cancer cells(0.408±0.004) and control cells [(0.388±0.005),F=22.052,P=0.002].In migration experiment,the number of migration cells in siRNA group,Lewis lung cancer cell group and control group were(31.0±10.5),(56.0±5.4) and(49.0±9.1) respectively,which were significantly different(F=11.213,P=0.002).In invasion experiment,the number of invasion cells in siRNA group(21.0±6.6) were lower compared with Lewis lung cancer cell group and control group [(59.0±9.8),(43.0±18.0)],which were also significantly different(F=11.692,P=0.002).CONCLUSION: Down-regulating the expression of Vav3 gene by siRNA can inhibit migration and invasion ability in Lewis lung cancer cells.