Genetic Transformation of Eucommia ulmoides Oliv. with the Late Embryogenesis Abundant Protein Gene

Eucommia ulmiodes Oliv. calli were induced on an MS medium supplemented with 2.0 mg l-1 2,4-dichlorophenoxy acetic acid (2,4-D), and a good suspension was obtained in a liquid medium after 3 rounds of subculturing. The suspension cells were transformed by particle bombardment using the pBY520 plasmid containing the late embryogenesis abundant protein gene from barley (Hordeum vulgare L.), namely, the LEA3 gene. After 4 rounds of selection culturing on a medium containing phosphinothricin, 338 resistant calli were obtained.