Glutamate synthase from Bacillussubtilis: In vitro reconstitution of an active amidotransferase

Summary In Bacillus subtilis , two mutants, designated gltA and gltB , have been found to lack the enzyme glutamate synthase. When extracts from these two mutants were mixed together, an active enzyme was reconstituted. Since modification of an inactive enzyme to an activated form does not seem likely, we propose that the gltA and gltB mutations affect the two non-identical subunits which form glutamate synthase.