Histological observation of eye ontogeny in rock bream larvae (Oplegnathus fasciatus)

Based on the histological observation under optical microscope,we studied the ontogeny of eyes in rock bream.The results showed that at 24℃±1.0℃,the optic vesicle appeared at 10.5 h after fertilization,and the lens was visible at 15.5 h after fertilization.As the choroid fissures widened,the cornea was developed in the site at 22 h after fertilization.At 2 DAH(days after hatching),the choroid and sclera were formed,and the iris was present at 3 DAH.At 6 DAH,the retina was well developed and consisted of pigment epithelium,rods and cones layer,external limiting membrane,outer nuclear layer,outer plexiform layer,inner nuclear layer,inner plexiform layer,ganglion cell layer,nerve fibre layer and internal limiting membrane.At 13 DAH,the cornea was well differentiated,and consisted of stratified squamous epithelium,lamina elastica anterior,fibre layer,lamina elastica porterior and endothelium.At 21 DAH,the iris was well developed and consisted of pigment layer,iris posterior,stroma,iris anterior and endothelium.At this time,the eyes of rock bream were developed completely.