分散固相萃取－超高效液相色谱－串联质谱法快速测定苹果中代森锰锌残留

It is difficult to directly determine mancozeb because they are insoluble in general solvents. And the residue analysis in agricultural and crops is more complicated currently. In this study, a rapid method has been developed for the determination of germicide mancozeb residues in apple. The mancozeb of apple sample, which was methylated by dimethyl sulfate, was extracted by the Quick, easy, cheap, effective, rugged and safe method and detected by ultra performance liquid chromatography-mass spectrometry under multiple reaction monitoring mode, then quantified by matrix-match standard solution. The qualitative results were obtained based on retention time, the precursor ion and two daughter ions, and the quantitation results were on the intension of the characteristic m/z 241/117 ion and m/z 241/193 ion. Average recoveries of man-cozeb in apple samples were found in the range of 88.7%-109.4% at four spiking levels from 0.005 to 0.5 mg/kg with relative standard deviations of 5.9%-8.7%. Limits of detection of mancozeb were 2.5×10^(-4)mg/kg, while limits of quantification were 8.3×10^(-4)mg/kg. The method is simple, rapid, and accurate, and can meet the requirements of the domestic and international legislation.