Calorimetric study on thermally-induced conformational change of dihydrofolate reductase

Dihydrofolate reductase(DHFR)(EC 1.5.1.3)exists in all biobodies.It is an importantcomponent of the chemistry of DNA synthesis in cells.Methotrexate,a potent inhibitor ofDHFR,is a well-known anticancer agent.It has been employed extensively in clinicaltreatment.Therefore,the study on the conformational change of DHFR gives occasion topeople’s close attention.The thermally-induced conformational change of DHFR was