The effects of Femarelle and Vitamin D Analog on Lipoxygenase Products and Reactive Oxygen Species (ROS) formation in the Female and Male Human-Derived Cultured Osteoblastic Cells

1. AbstractWe have previously reported that human primary cultured bone cells (hObs) respond to estradiol-17β (E2) and different phytoestrogens and vitamin D analogs by modulating cell proliferation. In the present study hObs derived from biopsies of pre- or post- menopausal females or males were cultured and treated with different estrogens and with the non-calcemic vitamin D JK-1624F2-2 (JKF) and assayed for the expression of 12LO and 15LO mRNA as well as for the synthesis of 12 and 15HETEs and the formation of ROS. All cells express 12LO and 15LO and produce 12 and 15 HETEs. Treatment with all estrogens: E2, daidzein (D) and femarelle (F) as well as JKF modulated the expression of both 12 and 15LO mRNA in pre- or post-menopausal female hObs and to some extent in male hObs. 12 and 15HETEs production was also modulated by these compounds in these cells. ROS formation was increased by all hormones in pre- and post- but not in male hObs. ROS formation was also increased by 12 and 15HETEs in pre- and post- ...