Detection of biodegradation of pyrene by synchronous fluorometry.

The synchronous fluorometry has been adopted to study the biodegradation rate of pyrene dissolved in mineral salts media (MSM). The limit of detection is calculated as 0.19ng/mL for the dissolved pyrene with the relative standard deviation less than 1.3% (n=9). The capability of three kinds of pyrene degrading strains has been investigated by the established method under same experimental condition. The results show that greatest feature of the process is its capability to detect directly without extraction. The result obtained is identical with the result of GC/FID experiment.