Connexin 43 gene expression in mice with cardiopulmonary developmental defects

1. Abstract 2. Introduction 3. Materials and Methods 3.1. Animals 3.2. Visualization of pulmonary arterial outflow 3.3. Light microscopy 3.4. cDNA microarrays 3.5. Extraction of Total RNA and Northern Blotting 3.6. Preparation of p probes 3.7. Quantitative Real Time RT-PCR 3.8. Western blotting 3.9. Immunohistochemistry 3.10. Statistical Analysis 4. Results 4.1. Mice with craniofacial defects 4.2. Lung morphology 4.3. Heart morphology 4.4. Heart histology 4.5. Identification of altered gene expressions by cDNA microarray 4.6. Cx43 mRNA expression 4.7. Quantitative Real time RT-PCR for Cx43 4.8. Cx43 protein levels 4.9. Immunohistochemical localization of Cx43 5. Discussion 5.1. Connexins: Cell Growth and Differentiation 5.2. Craniofacial Developmental Defects 5.3. Cardiac Developmental Defects 5.4. Cx43 modulations and Cardiopulmonary Abnormalities 5.5. Cx43 and ECM molecules 5.6. Significance of Cx43 6. Conclusions 7. Acknowledgments 8. References