Construction of the PEDV Adenovirus Vaccine and Mice Immune Experiment

sM,M,S gene of PEDV vaccine stain were implified by amplified by RT-PCR and reorganization PCR,then inserted into the pShuttle-CMV vector.These recombinant shuttle plasmids were linearized by PmeⅠand then transformed to BJ5183 cells containing the pAdEasy-1 bone plasmid.The homologous recombinantplasmidwas transformed to XL-10 Gold ultracompetent cells in order to obtain this plasmid in a large scale.These recombinant adenoviruses were co-infected Vero cells,mice immune experiment indicated that antibodies were induced by Western blotting.