Structural Studies on Induced Antibodies with Defined Idiotypic Specificities: VI. Amino Terminal Sequences of the Heavy and Light Chain Variable Regions of anti-p-azophenylarsonate Antibodies from A/J Mice Suppressed for a Cross-Reactive Idiotype

Previous studies in this series have been directed toward the elucidation of the heavy and light chain variable region structures of antibodies raised in A/J mice to the p -azophenylarsonate (Ar) hapten, certain of which bear a cross-reacting idiotype. The present study concerns an analysis of anti-Ar antibodies that arise in A/J mice suppressed for a cross-reacting idiotype. The results indicate that when an idiotype is suppressed and the animal subsequently hyperimmunized, the resultant antibodies are “deviated” into different V-region subgroups, both in the heavy and light polypeptide chains. The study presents the first primary structural analysis of a humoral immune response that has been manipulated by an idiotypic reagent.