Analytical and semipreparative separation of the enantiomers of new acetylcholinesterase inhibitors by high-performance liquid chromatography

The resolution of the enantiomers of new acetylcholinesterase inhibitors by high-performance liquid chromatography (HPLC) was investigated on stationary phases containing cellulose tris-(3,5 methylphenylcarbamide) (Chiralcel OD). The effects of the mobile phase on retention, enantioselectivity and resolution were also studied. Ethanol and isopropanol were tested as organic modifiers and the influence of diethylamine was investigated. The effect of temperature on chiral separations was also studieded.