Determination of carbendazim residue in Chinese chestnut by Reversed Phase High Performance Liquid Chromatography.

Residual carbendazim in Chinese chestnut was determined by Reversed-Phase High-Performance Liquid Chromatography(RP-HPLC).Samples was extracted with methanol,clean up with petroleum ether to remove fatty impurities,and further extracted with dichloromethane.Separation and purification was executed by Kromasil C18 column(250 mm×4.6 mm i.d.,5μm).Methanol and ammonium acetate(32:68,V/V) was used as the mobile phase with a flow rate of 1.0 mL/min.The detection wavelength was at 285 nm and the column temperature at 38℃.The linearity was good with the range of 0.05～5 mg/L(r=0.999 9).The recoveries of carbendazim in Chinese chestnut samples were 94.4%-103.0% with less than 2.3% of coefficient of variation,and the limit of detection was 0.01mg/kg.