Complete nucleotide sequence analysis of RNA2 and phylogenetic analyses of a paprika isolate of broad bean wilt virus in Korea.

An isolate of Broad bean wilt virus 2 (BBWV 2), which was isolated from paprika showed spotty fruits. Host range of BBWV 2 was nine species including Chenopodium amaranticolor , and could be characterized by the symptoms include chlorotic local and distortion on upper leaves of C. amaranticolor. Datura stramonium induced chlorotic spot on uninoculated upper leaves. The purified virus particles were isometric about 25 nm in diameter. The genomic RNA of this virus consisted of two components of about 6.3 kb and 4.5 kb molecules and coat protein contained two polypeptide species which calculated with about 42 kDa and 22 kDa. We determined the complete sequence of RNA2 from Broad bean wilt virus 2 isolate Pa. The complete nucleotide (nt) sequence of RNA 2 for BBWV 2-Pa was 3,597 nt in length. encoding a pupative movement protein (MP) and coat protein (CP). Between BBWV 2-Pa and other serotype II favaviruses, the overall amino acid similarities in the polyprotein coding regions was and 84%-93% for RNA2. In the 5′UTR, the nucleotide similarity was 65%-92%. For the 3′UTRs, they are 38%-60%, respectively. For the Genome organization of BBWV 2-Pa was similar to other comoviruses in the Comoviridae .