Effect of numerical aperture on signal level in cylindrical waveguide evanescent fluorosensors

The effect of numerical aperture on signal level from fluorescent substances or solutions in the evanescent zone of a cylindrical waveguide is analyzed. The analysis applies to the case in which the fluorescence is excited by the evanescent wave of a fiber and the fluorescence signal is that which tunnels back into the same fiber. The analysis is for two cases: bulk fluorescence and fluorescence of a thin film layer. Experimental results are also presented.