Cultivo de células mãe limbares em membrana amniótica para reconstrução de tecido corneano: uma comparação de dois métodos

Objective: To grow cells on amniotic membrane corneal limbus with two different methods to assess the confluence, viability and differentiation of both. Methods: LSC samples were obtained from 16 deceased donors. Additionally, six cultures were made from each sample, 3 by LSC suspension method and 3 by LSC explant method, all in triplicate. Furthermore, AMs without oral mucosal cells were used as negative control cultures. The cells were seeded with SHEM, 37°C, 5% CO 2 , 95% humidity. Moreover, the cultures