Silica coating of luminescent quantum dots prepared in aqueous media for cellular labeling

Abstract Silica coating is an effective approach for rendering luminescent quantum dots (QDs) with water dispersibility and biocompatibility. However, it is still challenging to prepare silica-coated QDs (QD@SiO 2 ) with high emission efficiency, small size and great stability in favor for bioapplication. Herein, we reported a modified Stober method for silica coating of aqueously-prepared CdTe/CdS QDs. With the coexistence of Cd 2+ and thioglycolic acid (TGA), a thin silica shell was formed around QDs by the hydrolysis of tetraethyl orthosilicate (TEOS). The resultant QD@SiO 2 with a small size (∼5 nm in diameter) exhibits significantly higher emission efficiencies than that of the initial QDs. Also, QD@SiO 2 has extraordinary photo and colloidal stability (pH range of 5–13, 4.0 M NaCl solution). Protected by the silica shell, the cytotoxicity of QDs could be reduced. Moreover, the QD@SiO 2 conjugated with folic acid (FA) presents high specific binding toward receptor-positive HeLa cells over receptor-negative A549 cells.