Preparation and chiral recognition of new chiral stationary phases derived from cellulose microspheres

A new way to prepare cellulose-type chiral stationary phases (CSPs) has been established in the present work. Cellulose microspheres with a volume-average diameter of 11.5 m were prepared by an emulsion-solidification method. Three new CSPs were obtained by crosslinking the cellulose microspheres with terephthaloyl chloride (TPC), and then modifying the crosslinked microspheres with 4-methylbenzoyl chloride, 3,5-dimethylbenzoyl chloride and 3,5-dichlorobenzoyl chloride, respectively. The microspheres and the CSPs were characterized by FT-IR, element analysis and scanning electronic microscopy (SEM). The chiral recognition ability of the CSPs was evaluated with high-performance liquid chromatography (HPLC). The chromatographic results demonstrate that the CSP prepared from 3,5-dichlorobenzoyl chloride possesses better chiral recognition ability compared with two other CSPs.