Inflammatoin, Oxidative Stress and Carbonyl Stress in Uremic Patients

Objective To evaluate the states of inflammation, oxidative stress and carbonyl stress in uremic patients and analyze their relationships. Methods One hundred and twenty-eight cases were divided into 6 groups: non-dialysis uremia group(n=25), peritoneal dialysis group(n=19), hemophan(Hem) membrane dialysis group(n=25), polyamide(PS) membrane dialysis group(n=25), diabetes with normal renal function group(n=23) and normal control group(n=11). Spectrophotometry and immune turbidimetry were used to measure the serum SOD, VitC, VitE, MDA and total carbonyl compounds (TCC) levels. Results Compared with non-uremia groups, the uremia groups had lower serum SOD, VitE and VitC levels, but higher CRP, MDA and TCC levels (P0.01). The peritoneal dialysis group had higher SOD, VitC, VitE levels in comparison with the Hem group, but did not differ from PS group in those levels (P0.05). The MDA and TCC levels in the peritoneal dialysis group were lower than those in the two hemodialysis groups, but there were no significant differences in CRP level between the groups. Compared with Hem group, the PS group had higher VitC, VitE levels, higher TCC clearance, and the same SOD, MDA, CRP and pre-dialysis TCC levels. When the variables were analyzed with TCC, the results of multi-variate regression showed that the standardized coefficients were MDA(0.727,P0.01), CRP(0.370,P0.01), SOD(0.192,P0.05), VitC (-0.153,P0.01), VitE(0.054,P=0.30) respectively. Conlusion Uremic patients are in inflammatory, oxidative-stress and carbonyl-stress states. Inflammation and oxidative stress are probably the important mechanism of carbonyl stress. It is not yet clear whether dialysis methods can influence uremic inflammatory, oxidative-stress or carbonyl-stress state.