Genetic and cDNA-AFLP analysis of abnormal spike development in common wheat

This study found some plants with abnormal ears in F2 generations of two hybrid combinations Zhoumai 16 / Yunong 2 593 and Yunong 2 593 / Xinmai 9. All ears of these abnormal plants were dysplastic,spikelets got longer and arranged spacing increased obviously,the number of spikelets cut down,anthers were shriveled and thin,filaments were sparse and could not elongate,which resulted in abnormal blossom,and the development of pistil was atrophic,and all the traits could be inherited stably in different generations. The ratio of normal plants and abnormal plants from F3∶ 4and F4∶ 5of two hybrid combinations were 3∶ 1,which showed that the abnormal ear development was controlled by a pair of recessive genes,instead of environment. We screened a total of 17486 transcript derived fragments( TDF) in which 20 TDFs successfully got through 109 pairs of markers for single ridge stage and two stages of normal and abnormal type ear differentiation by cDNA-AFLP technology. And then these TDFs after cloning and sequencing were analyzed by bioinformatics. The result showed that 10 TDFs were identified of high similarity to known function genes,which functions included stress responding,metabolism of cellular and organization,transport facilitation,expression regular and signal transduction,6 TDFs were identified of high similarity with unknown function or putative protein,and the re-maining 4 TDFs were found to be of no sequence similarity which might be some new genes. These results have important theoretical guiding significance for the study of wheat spike developmental regulatory genes and the reveal of wheat spike differentiation process.