Bio-guided anti-cariogenic and phytochemical valorization of Guiera senegalensis and Pseudocedrela kotschyi stem extracts

The objective of the present study is to carry out bio-guided phytochemical investigation of Guiera senegalensis and Pseudocedrela kotschyi stem extracts. The two plants are used as toothpicks for oral hygiene. The inhibition test revealed a bacteriostatic effect of hexane extract PK1 of P. kotschyi against Streptococcus mutans ATCC 25175 and Streptococcus salivarius ATCC 20560, two bacterial cariogenic strains, with a decrease in the number of bacterial colonies of 1 Log/control. The aqueous extract GS5 obtained from the stems of G. senegalensis is bactericidal, with total inhibition of S. salivarius ATCC 20560. The antimicrobial effect of the stem extracts from the two plants studied varies according to the plant species and the type of bacterial strain. The phytocompounds 8-Hydroxy-6,7-dimethoxy-3-methylisochroman-4-one, 1-(4-hydroxy-3-methoxyphe-nyl) propane-1,2-dione and (4E, 15E) -Nonadeca-4,15- dien-10-one were isolated, respectively from GS5A extracts of G. senegalensis and PK1 of P. kotschyi by normal column chromatography. Key words: Guiera senegalensis, Pseudocedrela kotschyi, stem extracts, Streptococcus mutans, Streptococcus salivarius, spectroscopic characterization.