Method for measuring related substances of Sofosbuvir tablet by using HPLC

The invention provides a method for measuring related substances of a Sofosbuvir tablet by using HPLC. The method is characterized in that in an anti-phase C18 column, a phosphoric acid solution or a mixed solution of the phosphoric acid solution and an organic phase is adopted to carry out gradient elution detection on a Sofosbuvir tablet test solution, wherein termination processing is carried out on silica gel particles of the anti-phase C18 column. The method provided by the invention can effectively separate and measure the related substances of the Sofosbuvir tablet, and also has the characteristics of being good in specificity, high in sensitivity, convenient, rapid, economical, and low in cost.