Identification of Lysis-Relevant Molecules on the Surface of CTL: Primary Screening of Monoclonal Antibodies for the Capacity to Block Cytolysis by Cloned CTL Lines

Cytolytic T lymphocytes (CTL) couple antigen recognition with cytolysis of the target cell. The recognition (conjugate formation) and lethal hit components can be distinguished operationally. Both the antigen recognition structure and the lethal hit activity are unidentified. It is possible that the antigen recognition structure on CTL is modified so as to possess this lethal hit activity (1). Implicit in the work described here is the not unreasonable assumption that the molecule(s) responsible for recognition and for the lethal hit exist on the surface of the cytolytic T lymphocyte.