Competitive enzyme immunoassay for antibodies to 3-Acetyllupeol in brazilian Vernonia scorpioides grown under domestication

bSUMMARY – A solid phase indirect competitive enzyme immunoassay using polyclonal IgY antibodies raised to 3Acetyllupeol (3-AL) was applied to quantify the content of 3-AL in crude extracts obtained from stalks, leaves and roots of Vernonia scorpioides domesticated in individual vessels containing brazilian yellow-red podsolic soil added of different combinations Ca (NO 3)2 and of CaHPO 4. Test sensitivity for 3-AL decreased more than five orders of magnitude as the number of 3-AL ligands on the processed plant material reduced antibody recognition of the 3-AL conjugated to a carrier protein. Soil CaHPO4 concentration was directly related to crescent root production of lupeol-acetate, suggesting a phosphoenzyme metabolic activation, warranting further studies on this region of synthesis. The technique is designed for qualitative and quantitative studies and allows ready identification of 3-AL. It may also be further applied in the study of other plant bioactive substances lending support to certify raw products from natural sources. GRAPHICAL ABSTRACT – A competitive ELISA was applied to quantify the triterpene named 3-Acetyllupeol (1), an