Cell suspension culture of Saussurea medusa in bioreactor

Cell suspension culture of Saussurea medusa Maxim. was established in a 2 L stirred bioreactor for flavonoids production. After 12 days of culture, the biomass was 10.35 g DW.L-1, containing 428.6 mg/L flavonoids, which was 4.14% of the biomass (dry weight) under the condition of 25 degrees C and light intensity lower than 5 mu mol.m(-2).s(-1). The utility pattern of sugar, change of dissolved oxygen anti oxygen uptake rate were also studied.