[Effect of endothelium-specific deletion of PTEN on hemangioblast development in mouse embryo AGM region].

This study was aimed to investigate whether endothelium-specific deletion of PTEN can affect hemangioblast development in the AGM region of mouse embryos.Based on Cre/loxP system,the Tie2CrePtenloxp/loxp and Tie2CrePtenloxp/wt mouse embryos were obtained.The genotype was identified by PCR.After treated with type Ⅰ collagenase,the AGM region was dispersed into single-cell suspension,and then was cultured in blast colony-forming cell(BL-CFC) media.The number of BL-CFC was counted 4 or 5 days later.The hematopoietic capacity of BL-CFC was detected in methylcellulose culture system and the endothelial potential was assessed by tube-like structure formation on Matrigel.The results showed that the number of BL-CFC in AGM region of Tie2CrePtenloxp/loxp mouse embryo decreased as compared with Tie2CrePtenloxp/wt embryo.Whereas the hematopoietic capacity of mutant BL-CFC was enhanced,the endothelial potential,as evaluated by tube-like structure formation in vitro,was significantly reduced.It is concluded that the endothelial PTEN is capable of exerting regulatory functions on both the numbers and the dual potential of hemangioblast in mouse AGM region.