Investigations on Blood Activities of Some Enzymes in Dogs After Acute Intoxication with the Carbamate Insecticide Carbofuran

Experiments for monitoring of changes in blood enzyme activities were carried out in dogs after acute intoxication with the carbamate insecticide carbofuran (Carbosan 35 СТ). The studies involved one control and 6 experimental groups of dogs (total n=42), treated once orally with increasing doses of the preparation via oesophageal probe: 0.525 mg/kg (experimental group I), 1.05 mg/kg (experimental group II), 2.1 mg/kg (experimental group III), 3.5 mg/kg (experimental group IV), 5.25 mg/kg (experimental group V) and 10.5 mg/kg (LD 50 ), (experimental group VI), corresponding to 1/20, 1/10, 1/5, 1/3, 1/2 and LD 50 , oral doses for albino rats. Blood samples were obtained from v. antebrachi cephalica or v. jugularis in the course of 3 consecutive days prior to the treatment (hours -48, -24 and 0) and on post treatment hours 1, 3, 5, 7, 24 and 48 from all groups for analysis of acetylcholinesterase (AChE), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), amylase (AMY), gamma-glutamyltransferase (g-GT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH). It was established that the tested carbamate insecticide provoked lower blood activity of AChE and increased the levels of ASAT, ALAT, AMY, g-GT, AP and LDH between post treatment hours 1 and 7; afterwards, the studied parameters regained the respective control values.