자동화장비를 이용한 단염기연쇄 분석법의 시험방법 유효화 및 비교분석

In case of familial searching with large database, common STRs cannot provide sufficient kinship index. Thus, new STR kits with additional STR loci are necessary in order to complement conventional analysis for more reliable forensic information. In this study, the PowerPlex Fusion System (Promega, Madison, WI, USA), the GlobalFiler PCR Amplification Kit (Applied Biosystems, Foster City, CA, USA), and the PowerPlex Y23 System (Promega) were tested for internal validation. Validations of these new STR kits include all of the studies required by SWGDAM and compared with three conventional kits of Identifiler Plus (Applied Biosystems), NGMSElect (Applied Biosystems) and Yfiler (Applied Biosystems), and tests were performed by using Tecan Freedom Evo automated liquid handling system (Tecan, Mannedorf, Switzerland). Studies included reproducibility, precision, sensitivity of detection, minimum detection threshold, system contamination, stochastic effect and concordance. These forensic parameters were used to assess suitability of the new kits for forensic genetic analysis. Validation studies demonstrate that new STR kits are robust, reliable, and sensitive methods for STR testing. Furthermore, concordance studies showed consistent results when comparing PowerPlex Fusion, GlobalFiler and PowerPlex Y23 System with other commercially available STR-genotyping systems and these new STR kits showed remarkable allele call rates in 60 years old skeletal samples. These results indicated that new STR systems are potentially informative and may prove useful in identification of missing casualties from the Korean War.