Granulomatous prostatitis linked to hla-drb1*1501

Methods: This is a single-institution, retrospective study from the historic records of 12 eligible patients, eight of whom agreed to participate. Genomic DNA was extracted from anticoagulated blood. Intermediate-resolution genotyping for HLA-A, B, and DRB alleles and high-resolution genotyping for select HLA DRB1 and DRB 3/4/5 alleles were performed by polymerase chain reaction amplification using group-specific probes and subsequent hybridizations of the amplicons with sequence specific oligonucleotide probe. High-resolution, allele-specific identification of HLA DR 15 was performed if patients were DR 15-positive by intermediate-resolution typing. Results: Seventy-five percent of white patients were HLA-DR 15-positive by intermediate-resolution typing versus 28.2% in the control group of 451 patients. Thirtythree percent of black patients were HLA-DR 15-positive compared with 25.8% of 89 black patients who served as the control group. The data suggest an association between HLA-DRB1*1501 in white men and granulomatous prostatitis. The data were consistent with a possible autoimmune etiology for nonspecific granulomatous prostatitis.