Power-free automated capillary flow assay for SARS-CoV-2 detection

An at-home serological test for SARS-CoV2 antibodies was developed that automatically performs sequential reagent delivery and washing for enzyme amplified colorimetric detection The device that houses the assay enables improved sensitivity and selectivity without sacrificing simplicity for untrained end-users The microfluidic device was made by laminating transparency sheets and double-sided adhesive and requires only two user steps to operate Once flow is initiated in the microfluidic channels, reagents are sequentially delivered to and washed from a detection zone, where a colored line indicates the presence of antibodies Anti-SARS-CoV-2 nucleocapsid protein has been detected from whole blood at clinically relevant concentrations © 2020 CBMS-0001