Effect of EG and DMSO on Spindle and Parthenogenetic Development of Matured Oocyte after Vitrification in Mice

The present study was designed to cryopreserve the mouse matured oocytes with vitrification solution EFS30,DFS30 or EDFS30,and then the survival,morphology of spindle after thawing.Moreover,the development potential in vitro after parthenogenetic activation were also detected.The results indicated that the rate of survival of oocytes after thawing in EDFS30 group (98.3%) were significantly higher than that of EFS30 (88.2%) and DFS30 group (89.5%)(P 0.05);The rate of oocytes with normal spindle in DFS30 group (44.9%) were significantly lower than that of other groups (P 0.05).However,there were no difference among each groups in not only the cleavage rate,blastocyst rate and the number of blastocysts after parthenogenetic activation.In conclusion,all of the vitrification solutions EFS30,DFS30 or EDFS30 could be used for mouse oocytes cryopreservation,but EDFS30 was more suitable.