Studies on optimizing extraction technics of flowers of Polygonum orientale by response surface methodology
2014
Objective: To analyze and optimize extraction technics of Polygonum orientale flowers by response surface methodology. Methods:With the index for the content of taxifolin in flowers of Polygonum orientale,the effect of three factors such as concentration of alcohol,extraction time and solvent-solid ratio was designed by Box-Behnken central composite. Extraction technic parameters of Polygonum orientale flowers was optimized by response surface methodology. Results:The optimizing extraction conditions of Polygonum orientale flowers were as follows:ethanol concentration was 65%,extracting time was 129 min and solvent-solid ratio was 18. Under the conditions,the average yield of taxifolin in 3 validation experiments was 2. 79 mg / g. Conclusion:Optimizing extraction technics by response surface methodology is reasonable,simple,and has good predictability.
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