全血処理型白血球除去フィルタークローズドバッグシステム(セパセルインテグラMAP)を用いた血液製剤の調製と長期保存試験

A newly developed leukoreduction filter system for whole blood was evaluated. Whole blood was leukoreduced with the closed system (Sepacell Integra MAP), followed by centrifugation to obtain red cells and plasma (Leukocyte-reduced plasma: LR-FFP).Red cells were reconstituted with MAP solution (Leukocyte-reduced RCC: LR-RCC).Residual leukocyte counts were 1.8±1.3×105/bag after filtration and 0.9±0.5×105/bag in LR-RCC. These values are well below those indicated in the AABB standard and CE guideline. Erythrocyte recovery rate in Sepacell MAP was 89.1±2.3%, which was not different from that in red cell concentrate without filtration (buffy coat reduced RCC: BCR-RCC).There was no accumulation of IL-1β, IL-8 or LDH in the supernatants of LR-RCC during storage.Further, there was no significant difference in electrolyte levels in the supernatant and red cell fractions during storage between LR-RCC and BCR-RCC.In LR-FFP, elevation of C3a was observed in 2 of 6 samples, while elevation of LDH was not observed.