Novel Biotin Phosphoramidites with Super-Long Tethering Arms

Abstract A number of novel biotin phosphoramidites, possessing exceptionally long and uncharged tethering arms, were synthesized from methoxyoxalamido (MOX) and succinimido (SUC) precursors. Included among these monomers is a uridine derivative with the biotin moiety attached through the 2′-position. Some of these phosphoramidites were used to make 5′-biotinylated primers, which were applied in direct sequencing of genomic DNA and capture of Sanger fragment pools.