Application of luminescence spectral assay for monitoring of intracellular metabolism in hepatocytes

The method of evaluation of intracellular metabolism level in chickens' hepatocytes, using luminescence spectral analysis with 5-([4.6-Dichlorotriazin-2-yl]amino) fluorescein hydrochloride (DTAF) is proposed. The dynamics of proteins content in chickens' hepatocytes under experimental escherichiosis and in intact poultry was established using this method. A gradual increase in the proteins content in hepatocytes of intact chickens was revealed from 1 to 30 days. The proteins content in hepatocytes of chickens with experimental escherichiosis was gradually increased during the monitoring period, to the exclusion of the sixth day indicators, which were kept at fourth day level (4th day - 2.15 ± 0.01; 6th day – 2. 15 ± 0.02). Moreover, these indicators were less than similar indicators of the control group throughout the experiment.