Encapsulation of alkaline phosphatase in polyacrylamide microparticles using the concentrated emulsion polymerisation method

Polyacrylamide microparticles were prepared by the concentrated emulsion polymerisation technique. In distilled water the microparticles have spherical form and two size distributions with average diameters 1.5 μm and 5.7 μm. Alkaline phosphatase was encapsulated in the microparticles. The encapsulation of the enzyme slightly alters the size of the new microparticles that present average sizes of 1.4 μm and 6.2 μm. However, the activity of the enzyme is reduced to 12% as compared with the free enzyme.