Microwave Extraction Followed by Solid-phase Extraction for the Determination of Phthalate Esters in Fish Samples by Gas Chromatography

A new analytical procedure based on solid-phase extraction method coupled to GC-FID has been developed and validated for the determination of five phthalate esters (PAEs) (dimethyl-(DMP), diethyl-(DEP), di-n-butyl-(DBP), di-2-ethylhcxyl-(DEHP), di-n-octyl-(DOP)) in fish samples. There was a good linear relationship in the range of 0.05-10ig with the detection limits of 0.09-0.16ng. The recoveries obtained for PAEs ranged from 88.6% to 96.4% with RSD of 4.2%-10.2%. The applicability of the developed method was demonstrated for real fish samples.