Phylogenetic andMolecular Characterization ofa 23SrRNA GenePositions theGenusCampylobacter intheEpsilon Subdivision oftheProteobacteria andShowsthatthePresence ofTranscribed Spacers IsCommoninCampylobacter spp.t

1605 AirThermo-Cycler from Idaho Technology (Idaho Falls, Idaho)essentially as described by Gustafson et al. (14). The samplesweresubjected to 35 cycles of 1 s ofdenaturation at 94°C, 1 sof primer annealing at 51°C, and extension at 71°C for 40 s.PCR-generated DNAproducts were detected by gel electro-phoresis on 1.0% agarose. DNAfragmentswerevisualized byUVfluorescence after ethidium bromide staining. APCRcontrol was routinely included with every primer pair. Thiscontrol reaction contained water instead of DNAto detectunwanted DNAcontaminants.RNAextraction andgel electrophoresis. RNAwaspreparedas described by Burgin et al. (3). Cells grown for 24 h werewashedoffMueller-Hinton agar plates with Tris HCl(50 mM,pH8.0). Following centrifugation at 4°C, the cell pellet wasresuspended in 3 ml of Tris HCl (50 mMpH 8.0)-2 mM