Dispersive Pipette Extraction (DPX) of Benzoylecgonine in Urine with analysis by LDTD-MS/MS

Analysis of certain drugs of abuse in urine can require a sample clean-up step to reduce the interference effect from the matrix. To obtain an optimal sample clean-up, Dispersive Pipette eXtraction (DPX) technology is used. This technology uses a pipette tip to provide fast and efficient sample clean-up with extractions in less than 1 minute. The extraction is simply aspirate and dispense using a pipette tip containing loose sorbent. The tips can be used with automated liquid handling systems or with a semiautomated extractor. The DPX Polar tips are used in the extraction procedure prior to the Laser Diode Thermal Desorption (LDTD) analysis. The LDTD ion source uses an infrared laser diode to desorb samples that have been previously dried onto a 96-well LazWellTM plate after sample preparation extraction. The rapid desorption produces neutral species, which are carried into a corona discharge region to undergo an efficient protonation and are subsequently transferred directly into the mass spectrometer for detection.