PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone

To establish a convenient and rapid method for extracting DNA from bone.Fifteen long bone samples were washed and sterilized. The skeletal fragments were obtained by electric drill, and lysed by PrepFiler Express BTA™ lysis buffer. DNA was then manually extracted by silicon microbeads for further analysis.STR genotyping was successfully obtained in 14 out of the 15 samples, and the detection rate was 93.33%.The method for DNA extraction from bone established in present study is convenient, quick, effective, and with a strong applicability, which is worth spreading and applying.